Year 2015, Volume 32 , Issue 1, Pages 47 - 62 2015-01-16

Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers
Valencia ve Navel grup portakal çeşitlerinin SSR markörleri yardımıyla genetik çeşitlilik analizi

İlknur POLAT [1]


Sweet orange [Citrus sinensis (L.) Osbeck] fruit is one of the main citrus fruits, Navel and Valencia group sweet orange being the most representative and recognizable species of this species. The aims of this study were to determine genetic relationships and diversity of 84 Navel and 36 Valencia groups of sweet orange using SSR (simple sequence repeat) molecular markers. Twenty-six SSR primers were tested on these accessions. Seven SSR primers produced thirteen polymorphic fragments, eight SSR primers produced monomorphic fragments, and eleven SSR primers produced no scorable fragments. Thirteen SSR primers produced a total of 29 fragments and 13 of them were polymorphic. The number of average polymorphic fragments per primer was 1.93. The mean polymorphism information content (PIC) and marker index (MI) are 0.16 and 11.74, respectively. The Dice’s similarity coefficient among Navel and Valencia group sweet oranges ranged from 0.42 to 1.00 and matrix correlation (r) was 0.79. In the cluster analysis, Navel group sweet oranges were indicated as a separate group from Valencia group sweet oranges. ‘Antalya (40)’ was most distinct accessions from the others.

Portakal [Citrus sinensis (L.) Osbeck] turunçgil yetiştiriciliği içerisinde en önemli türü oluşturur, Navel ve Valencia grubu portakallar ise portakallar içerisinde en fazla yetiştirilenlerdir.  Çalışmanın amacı, 84 adet Navel ve 36 adet Valencia grubu portakalın SSR (simple sequence repeat) moleküler markırı kullanarak genetik akrabalıklarını ve farklılıklarını belirlemektir. Kullanılan 26 SSR primerinden 13 tanesi polimorfik, 8 tanesi monomorfik bant sağlarken, 11 primerden değerlendirilebilecek bant elde edilememiştir. Polimorfizm sağlayan 13 SSR primerinden toplam 29 bant elde edilmiş ve bunların 13 adeti polimorfizm sağlamıştır. Her bir primere düşen ortalama polimorfik bant sayısı 1.93’tür. Ortalama PIC (polymorphism information content) ve MI (marker index) değerleri sırasıyla 0.16 ve 11.74’dür. Navel ve Valencia grubu portakallarında Dice’ın benzerlik indisi (Dice’s similarity coefficient) 0.42 ile 1.00 arasında değişim göstermiştir, matriks korelasyon (r=matrix correlation) ise 0.79’dur. Kümeleme analizinde (cluster analysis) ise, Navel grubu portakallar Valencia grubu portakallardan ayrılmıştır. ‘Antalya (40)’ tüm portakal grupları içerisinde en uzak bireyi oluşturmuştur.
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Primary Language en
Journal Section Research Articles
Authors

Author: İlknur POLAT

Dates

Publication Date : January 16, 2015

Bibtex @ { derim62691, journal = {Derim}, issn = {1300-3496}, eissn = {2149-2182}, address = {}, publisher = {Batı Akdeniz Agricultural Research Institute}, year = {2015}, volume = {32}, pages = {47 - 62}, doi = {10.16882/derim.2015.93793}, title = {Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers}, key = {cite}, author = {POLAT, İlknur} }
APA POLAT, İ . (2015). Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers. Derim , 32 (1) , 47-62 . DOI: 10.16882/derim.2015.93793
MLA POLAT, İ . "Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers". Derim 32 (2015 ): 47-62 <http://www.derim.com.tr/en/issue/4578/62691>
Chicago POLAT, İ . "Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers". Derim 32 (2015 ): 47-62
RIS TY - JOUR T1 - Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers AU - İlknur POLAT Y1 - 2015 PY - 2015 N1 - doi: 10.16882/derim.2015.93793 DO - 10.16882/derim.2015.93793 T2 - Derim JF - Journal JO - JOR SP - 47 EP - 62 VL - 32 IS - 1 SN - 1300-3496-2149-2182 M3 - doi: 10.16882/derim.2015.93793 UR - https://doi.org/10.16882/derim.2015.93793 Y2 - 2019 ER -
EndNote %0 Derim Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers %A İlknur POLAT %T Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers %D 2015 %J Derim %P 1300-3496-2149-2182 %V 32 %N 1 %R doi: 10.16882/derim.2015.93793 %U 10.16882/derim.2015.93793
ISNAD POLAT, İlknur . "Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers". Derim 32 / 1 (January 2015): 47-62 . https://doi.org/10.16882/derim.2015.93793
AMA POLAT İ . Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers. DERİM. 2015; 32(1): 47-62.
Vancouver POLAT İ . Genetic diversity analysis of Valencia and Navel group sweet orange cultivars by SSR markers. Derim. 2015; 32(1): 62-47.